synoviocytes  (Cell Applications Inc)

Journal: bioRxiv

Article Title: A human synovial tendon-on-a-chip models key features of peritendinous adhesions and offers a new approach methodology for testing anti-fibrotic drugs

doi: 10.64898/2026.04.03.716316

Figure Lengend Snippet: a , Immunofluorescence images of HUVEC cultured in monolayer and stained for VE-cadherin (green) and Hoechst (blue) compare four different media conditions at 24 h and 72 h of culture. b, Mean fluorescence intensity (MFI) of VE-cadherin expression at b, 24 h and c, 72 h, normalized to MFI of EGM-2 images. Synoviocyte growth media had significantly less VE-cadherin expression at 24 h compared to EGM-2. VE-cadherin MFI for 1:1 Synoviocyte Growth Media:EGM-2 most closely matched that of EGM-2 conditions. d, Immunofluorescence images of FLS cultured in a monolayer, stained for cadherin-11 (yellow) and Hoechst (blue) compare the four different media conditions at 24 h and 72 h of culture. e, MFI quantification of cadherin-11 expression, normalized to MFI of Synoviocyte Growth Media, demonstrated no significant differences between culture conditions at 24 h. f, At 72 h, EGM-2 and 1:1 Synoviocyte Growth Media:EGM-2 had significantly higher cadherin-11 expression compared to Synoviocyte Growth Media, while DMEM had comparable levels. One-way ANOVA with Tukey’s post-hoc test: n=3 wells per condition, *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: FLS were expanded in T-75 flasks in Synoviocyte Growth Medium (Cell Applications, 415K-500).

Techniques: Immunofluorescence, Cell Culture, Staining, Fluorescence, Expressing

Journal: bioRxiv

Article Title: Characterization of emerging Oropouche virus tropism and pathogenicity

doi: 10.64898/2026.03.25.714204

Figure Lengend Snippet: A: dPCR results of human chondrocytes (HC, left), Fibroblast-Like Synoviocytes (HFLS, middle) and muscular cells (CHQ; right) cultures supernatants infected with OROV at MOI 0.1 and collected at different times (24, 48, 72 h p.i.). The graphs show 18 points (except HFLS with 12 points) per condition, as well as the mean and geometric standard deviation. ns: not significant; *: p-value < 0.05; **: p-value < 0.01; ***: p-value < 0.001; ****: p-value < 0.0001 (Dunn-Bonferroni non-parametric test). B: HC (left), HFLS (middle), and CHQ (right) cells were infected with OROV at different MOIs. Cell lysates were collected at 48 or 72h p.i. and analysed by Western blot, using antibodies against calnexin (CNX) as internal control and against the N protein of OROV (OROV N). C: The supernatants from HC (left), HFLS (middle) and CHQ (right) cultures were collected at different times after infection with OROV at MOI 0.1. The viral titer was determined on Vero cells using xCELLigence technology from a standard range. Each condition has 6 replicates. ns: not significant; *: p-value < 0.05; **: p-value < 0.01 (Dunn-Bonferroni non-parametric test).

Article Snippet: Human chondrocytes (HC) and synoviocytes (HFLS) were obtained from Cell Applications, (Ref PB-402-05a and 408K-05a, respectively) and cultured according to manufacturer’s instructions.

Techniques: Infection, Standard Deviation, Western Blot, Control

Journal: bioRxiv

Article Title: Characterization of emerging Oropouche virus tropism and pathogenicity

doi: 10.64898/2026.03.25.714204

Figure Lengend Snippet: HC, HFLS and CHQ cells were infected on coverslips with OROV at MOI 0.1. Gc protein of the OROV virus was detected (green), and nuclei were also labelled (blue). The scale bar represents 200 µm.

Article Snippet: Human chondrocytes (HC) and synoviocytes (HFLS) were obtained from Cell Applications, (Ref PB-402-05a and 408K-05a, respectively) and cultured according to manufacturer’s instructions.

Techniques: Infection, Virus

Journal: Communications Biology

Article Title: METTL3-mediated fibroblast-like synoviocytes senescence promotes temporomandibular joint osteoarthritis progression

doi: 10.1038/s42003-026-09773-x

Figure Lengend Snippet: BLM-treated hFLSs were transfected with shMETTL3-1 (IME4-1) or shMETTL3-2 (IME4-2). A , B The transfection efficiency of shMETTL3-1 and shMETTL3-2 was verified by Western blotting. C , D Verification of cellular senescence with a β-GAL kit. E TEM for observing mitochondrial autophagosomes. F , G TMRM staining. H , I Western blotting data of P62 expression in the extracted mitochondria. J – P Western blotting analysis of p16 INK4a , p21, DcR2, PINK1, and Parkin. Q Double IF staining results for LC3B and TOMM20. *** P < 0.001 vs. the CTRL group; ## P < 0.01, ### P < 0.001 vs. the BLM+shCTRL group.

Article Snippet: Human fibroblast-like synoviocytes (hFLSs) were obtained from Cell Applications, Inc. (No. 408-05a, San Diego, CA, USA) and maintained in HFLS medium (No. 415–500, Cell Applications) at 37 °C with 5% CO2, as previously described .

Techniques: Transfection, Western Blot, Staining, Expressing

Journal: Communications Biology

Article Title: METTL3-mediated fibroblast-like synoviocytes senescence promotes temporomandibular joint osteoarthritis progression

doi: 10.1038/s42003-026-09773-x

Figure Lengend Snippet: The m 6 A levels of PINK1 mRNA were confirmed by m6A RIP-qPCR in the synovial tissues of sham and MIA-induced TMJOA rats ( A ), primary FLSs from sham and MIA-induced TMJOA rats ( B ), BLM-treated hFLSs ( C ), and METTL3-silenced hFLSs after treatment with BLM ( D ), ** P < 0.01; *** P < 0.001. E Potential m6A sites for PINK1 mRNA. MeRIP-qPCR analysis of PINK1 m 6 A mRNA levels at the 2028 ( F ), 2074 ( G ), 2174 ( H ), and 2483 ( I ) sites in hFLSs after transfection with shMETTL3 (IME4), ** P < 0.01; *** P < 0.001. J After IME4 silencing, the level of PINK1 was monitored via RT-qPCR in BLM-induced hFLSs treated with 2.5 μM actinomycin D for 0, 4, 8, and 12 h. The estimated half-life of PINK1 mRNA was 7.83 h in the BLM group and 11.33 h after IME4 knockdown. Data are mean ± SD from three independent experiments. *** P < 0.001 vs. the CTRL group; ### P < 0.001 vs. the BLM group.

Article Snippet: Human fibroblast-like synoviocytes (hFLSs) were obtained from Cell Applications, Inc. (No. 408-05a, San Diego, CA, USA) and maintained in HFLS medium (No. 415–500, Cell Applications) at 37 °C with 5% CO2, as previously described .

Techniques: Transfection, Quantitative RT-PCR, Knockdown

Journal: Communications Biology

Article Title: METTL3-mediated fibroblast-like synoviocytes senescence promotes temporomandibular joint osteoarthritis progression

doi: 10.1038/s42003-026-09773-x

Figure Lengend Snippet: BLM-induced hFLSs were transfected with shMETTL3 or shPINK1. A , B Validation of PINK1 protein expression. C , D Assessment of cellular senescence with a β-GAL kit. E TEM images. F , G TMRM staining. H , I P62 expression in mitochondria. J – M Western blotting analysis of Drp1, Mfn2, p16 INK4a , p21, and DcR2 expression. N Double IF staining of LC3B and TOMM20 expression. *** P < 0.001.

Article Snippet: Human fibroblast-like synoviocytes (hFLSs) were obtained from Cell Applications, Inc. (No. 408-05a, San Diego, CA, USA) and maintained in HFLS medium (No. 415–500, Cell Applications) at 37 °C with 5% CO2, as previously described .

Techniques: Transfection, Biomarker Discovery, Expressing, Staining, Western Blot